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KMID : 0043320010240040342
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Archives of Pharmacal Research
2001 Volume.24 No. 4 p.342 ~ p.348
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Apoptosis-Inducing Costunolide and a Novel Acyclic Monoterpene from the Stem Bark of Magnolia sieboldii
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Park HJ
Kwon SH/Han YN/Choi JW/Miyamoto KI/Lee SH/Lee KT
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Abstract
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In a course of obtaining more amount of bioactive costunolide and successive phytochemical isolation from Magnolia siebodiil (Magnoliaceae), a novel acyclic monoterpene 1 named deoxygeraniol (2,6(f)-dimethyl-2,6-octadiene) was isolated along with 7-sitosterol 3-0-linoleate (2), trilinolein (3) and high amount of costunolide (4) in the pure state. The structure of compound 1 was determined on the basis of spectroscopic data. Costunolide was found to Induce apoptotic cell death in a dose- dependent manner by nucleosomal DNA ladder and flow cytometric analysis. Immunoblot analysis showed that the level of the anti-apoptotic protein, Bcl-2, was decreased, whereas the cleavage of poly-(ADP-ribose) polymerase was activated. Furthermore, the N-acety1-L-cystei no antioxidant effectivel Prevented costu nolide-induced cytotoxicity. These results suggest that costunolide-induced cell death is mediated by reactive oxygen species.
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KEYWORD
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